Fiorella Infascelli was born in Rome. She started her career as a photographer for important magazines, such as Mondo and Espresso.

After that she approached the world of cinema and started working as second assistant and then as first assistant director for Giuseppe Bertolucci in Berlinguer ti voglio bene (Berlinguer, I Love You), Pier Paolo Pasolini in Salò e le 120 giornate di Sodoma (Salò, or the 120 Days of Sodom) and Bernardo Bertolucci in La tragedia di un uomo ridicolo (Tragedy of a Ridiculous Man).

In the ’80s, she started her career as a director with the film Ladra di Sogni, a silent comedy set in the XVIII century starring Roberto Benigni. In 1988, she shot her first feature film for cinema, La Maschera, a moral tale set in Italy in the XVIII century, starring Helena Bonham Carter and Michael Maloney. It was shortlisted for the award “Un Certain Regard” at Cannes, and won the Public Prize at the Annecy Festival.  Other important works ensued, such as Zuppa di Pesce (1992), Italiani (1998), Conversazione Italiana (1999), Ferreri I love you (2000) and Il vestito da Sposa (2003).

In 2011, she made the documentary Pugni chiusi about the workers of the Porto Torres petrochemical plant who, following the closure of the Vinils, decided to lock themselves in the former prison of the Asinara island for a year and a half. The film, screened at Venice in the Controcampo Italiano section, was awarded Best Documentary prize and the Osella prize for Best Cinematography.

Her last film, Era d’Estate, shot in 2015, is about the strange summer spent by Giovanni Falcone and Paolo Borsellino, together with their families, on the Asinara island to escape an attack. The film was broadcast by Rai Uno in May 2017 and was a success with both critics and the public.

During her career Fiorella Infascelli curated many different projects, including a series of interviews/portraits for various TV channels – including those with Roberto Benigni, Vincenzo Cerami, Mario Martone, and Luis Bacalov – two short films and some commercials for Pavesi, Corriere della Sera and Laura Biagiotti.